DNA SEQUENCING

We generate more than 1,000 bases of clean and edited data for plasmid and PCR DNA. View a typical analysis of a sample of sequences we produce in our facility. Each run is done with a control pGEM plasmid DNA to ensure accuracy of our results. With this high success rate, and method in place you are likely to receive all the data in just few days after sending in your samples. Continue reading

CFAR LABS offers you with Single Pass DNA Sequencing service for PCR DNA, Plasmid, and large-insert templates.

DNA Sequencing Service Description:

  • DNA sequencing reactions using BigDye terminator version 3.1
  • Clean-up of PCR products using QIAquick PCR purification kit from QIAGEN or streptavidin,R-phycoerythrin conjugate (SAPE)
  • Analysis of the extension products using ABI 3730XL
  • Data review and editing for every sample
  • Extensive trouble shooting for problematic samples
  • Customer support 24/7
  • Repeat Policy: If the first reaction does not produce usable data, the reaction will be repeated under modified reaction conditions at no extra charge.

Quality

We generate more than 1,000 bases of clean and edited data for plasmid and PCR DNA. View a typical analysis of a sample of sequences we produce in our facility. Each run is done with a control pGEM plasmid DNA to ensure accuracy of our results. With this high success rate, and method in place you are likely to receive all the data in just few days after sending in your samples.

Primer Banking

For your convenience upon your request, we bank your specific internal primers for future use at no extra charge.

Hard To Sequence Samples

We have extensive experience with high GC DNA, AT rich DNA, DNA with secondary structures, DNA with repetitive sequences, and dirty DNA, plasmid cloned in Gateway vector, etc. Trouble-shooting or problem solving support is available from our experienced staff.

DNA Sequencing Service Description:

  • Analysis of your reactions on an ABI 3730XL or 3730.
  • A thorough examination of the chromatograms and data editing.
  • Data review of each sample and return of review summary.

Plasmid DNA Preparation From Bacterial Clones

We purify your DNA in a single tube or 96-well plate for your convenience and perform DNA sequencing.
Gel or Column Purification of PCR Products
For your unpurified PCR product, we will column purify it prior to sequencing if your PCR amplification product contains a single robust band. If it contains multiple bands, gel purification of the band with the desired size will be performed.

Free DNA Sequencing Sample Shipping

You can drop off your DNA Sequencing samples at our facility located at Joint clinical research center, Lubowa Hill on Entebbe road.